A methodology for investigating protein adhesion and adsorption to microarrayed combinatorial libraries
Taylor, Michael and Urquhart, Andrew J. and Anderson, Daniel G. and Williams, Philip M. and Langer, Robert S. and Alexander, Morgan R. and Davies, Martyn C. (2008) A methodology for investigating protein adhesion and adsorption to microarrayed combinatorial libraries. Macromolecular Rapid Communications, 29 (15). pp. 1298-1302. ISSN 1022-1336 (http://dx.doi.org/10.1002/marc.200800171)
Full text not available in this repository.Request a copyAbstract
Here, we report the use of fluorescently labelled proteins to study protein adsorption to microarrayed synthetic polymers for the first time, indicating that this method is appropriate for the study of protein adsorption on these arrays. To investigate protein adhesion directly we use atomic force microscopy (AFM) to measure the force of adhesion between a protein-coated probe and the arrayed polymers. Both approaches show promise as methods for screening protein interactions with polymers in a microarray format. Comparison of these very different measures of protein-surface interactions indicate a good correlation.
-
-
Item type: Article ID code: 13155 Dates: DateEventJune 2008PublishedSubjects: Medicine > Therapeutics. Pharmacology
Medicine > Pharmacy and materia medica
Science > MicrobiologyDepartment: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Ms Ann Barker-Myles Date deposited: 06 Oct 2009 10:47 Last modified: 08 Apr 2024 17:04 URI: https://strathprints.strath.ac.uk/id/eprint/13155