Delivery of antimicrobials into parasites

Samuel, B.U. and Hearn, B. and Mack, D. and Wender, P. and Rothbard, J. and Kirisits, M.J. and Mui, E. and Wernimont, S. and Roberts, C.W. and Muench, S.P. and Rice, D.W. and Prigge, S.T. and Law, A.B. and McLeod, R. (2003) Delivery of antimicrobials into parasites. Proceedings of the National Academy of Sciences, 100 (24). pp. 14281-14286. ISSN 1091-6490 (http://dx.doi.org/10.1073/pnas.2436169100)

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Abstract

To eliminate apicomplexan parasites, inhibitory compounds must cross host cell, parasitophorous vacuole, and parasite membranes and cyst walls, making delivery challenging. Here, we show that short oligomers of arginine enter Toxoplasma gondii tachyzoites and encysted bradyzoites. Triclosan, which inhibits enoyl-ACP reductase (ENR), conjugated to arginine oligomers enters extracellular tachyzoites, host cells, tachyzoites inside parasitophorous vacuoles within host cells, extracellular bradyzoites, and bradyzoites within cysts. We identify, clone, and sequence T. gondii enr and produce and characterize enzymatically active, recombinant ENR. This enzyme has the requisite amino acids to bind triclosan. Triclosan released after conjugation to octaarginine via a readily hydrolyzable ester linkage inhibits ENR activity, tachyzoites in vitro, and tachyzoites in mice. Delivery of an inhibitor to a microorganism via conjugation to octaarginine provides an approach to transporting antimicrobials and other small molecules to sequestered parasites, a model system to characterize transport across multiple membrane barriers and structures, a widely applicable paradigm for treatment of active and encysted apicomplexan and other infections, and a generic proof of principle for a mechanism of medicine delivery.

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