Ca2+-sensitive and Ca2+-insensitive exocytosis in maize coleoptile protoplasts

Thiel, G and Sutter, J U and Homann, U (2000) Ca2+-sensitive and Ca2+-insensitive exocytosis in maize coleoptile protoplasts. Pflugers Archiv - European Journal of Physiology, 439 (3 Supp). R152-3. ISSN 0031-6768 (https://doi.org/10.1007/s004240000127)

Full text not available in this repository.Request a copy

Abstract

Ca2+ and osmotic driven extension of the surface area of maize coleoptile protoplasts was investigated using capacitance measurements and photolysis of the caged compound DM-nitrophen. Protoplasts responded to an elevation of cytoplasmic Ca2+ (Ca(i)) with a rapid burst in capacitance reaching a maximal increase of 1.3+/-1.1% over the resting cell capacitance. Subsequent lowering of the osmotic potential in the external medium by 210 mosmol caused a further increase in Cm by 26+/-6%. These data indicate two independent pathways for insertion of membrane into the plasma membrane. One is driven by Ca(i) and recruits membrane from a small pool. The osmotic evoked rise in surface area draws membrane from a much larger reservoir and may be driven by membrane tension.

  • Item type:Article
    ID code:39686
    Dates:
    Date
    Event
    2000
    Published
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:12 May 2012 10:23
    Last modified:08 Apr 2024 19:55
    URI:https://strathprints.strath.ac.uk/id/eprint/39686
CORE (COnnecting REpositories)