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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including those from the School of Psychological Sciences & Health - but also papers by researchers based within the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

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Human serum albumin and quercetin interactions monitored by time-resolved fluorescence : evidence for enhanced discrete rotamer conformations

Rolinski, O.J. and Martin, A. and Birch, D.J. (2007) Human serum albumin and quercetin interactions monitored by time-resolved fluorescence : evidence for enhanced discrete rotamer conformations. Journal of Biomedical Optics, 12. ISSN 1083-3668

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Abstract

Human serum albumin (HSA) complexation with quercetin, a flavonoid commonly present in human diet, was monitored by means of fluorescence decays of the single HSA tryptophan - Trp214. Data analysis based on fitting to multiexponential functions and determining the lifetime distributions revealed a high sensitivity of tryptophan fluorescence to binding quercetin. Results are discussed in terms of the rotamer model for tryptophan, HSA-quercetin complexation and potential HSA to quercetin energy transfer. Evidence for quercetin stabilising tryptophan rotamers in HSA is presented.