Rolinski, O.J. and Martin, A. and Birch, D.J. (2007) Human serum albumin and quercetin interactions monitored by time-resolved fluorescence : evidence for enhanced discrete rotamer conformations. Journal of Biomedical Optics, 12. 034013. ISSN 1083-3668
Full text not available in this repository. (Request a copy from the Strathclyde author)Abstract
Human serum albumin (HSA) complexation with quercetin, a flavonoid commonly present in human diet, was monitored by means of fluorescence decays of the single HSA tryptophan - Trp214. Data analysis based on fitting to multiexponential functions and determining the lifetime distributions revealed a high sensitivity of tryptophan fluorescence to binding quercetin. Results are discussed in terms of the rotamer model for tryptophan, HSA-quercetin complexation and potential HSA to quercetin energy transfer. Evidence for quercetin stabilising tryptophan rotamers in HSA is presented.
| Item type: | Article |
|---|---|
| ID code: | 9939 |
| Keywords: | proteins, molecular biophysics, fluorescence, biochemistry, molecular configurations, radiative lifetimes, rotational isomerism, human serum albumin, tryptophan rotamers, flavonoids, fluorescence lifetime distribution, fluorescence resonance energy transfer, quercetin, Physics |
| Subjects: | Science > Physics |
| Department: | Faculty of Science > Physics |
| Related URLs: | |
| Depositing user: | Strathprints Administrator |
| Date Deposited: | 02 Dec 2011 11:30 |
| Last modified: | 12 Mar 2012 10:53 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/9939 |
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