Graham, D. and Mallinder, B.J. and Smith, W.E. (2000) Detection and identification of labeled DNA by surface enhanced resonance Raman scattering. Biopolymers, 57 (2). pp. 85-91. ISSN 0006-3525
Full text not available in this repository. (Request a copy from the Strathclyde author)Abstract
The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 x 10(-12)M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis.
| Item type: | Article |
|---|---|
| ID code: | 961 |
| Keywords: | labeled DNA, surface enhanced resonance Raman scattering, Chemistry |
| Subjects: | Science > Chemistry |
| Department: | Faculty of Science > Pure and Applied Chemistry |
| Related URLs: | |
| Depositing user: | Mr Derek Boyle |
| Date Deposited: | 05 May 2006 |
| Last modified: | 12 Mar 2012 10:36 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/961 |
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