Graham, D. and Mallinder, B.J. and Smith, W.E. (2000) Detection and identification of labeled DNA by surface enhanced resonance Raman scattering. Biopolymers, 57 (2). pp. 85-91. ISSN 0006-3525Full text not available in this repository. (Request a copy from the Strathclyde author)
The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 x 10(-12)M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis.
|Keywords:||labeled DNA, surface enhanced resonance Raman scattering, Chemistry, Biochemistry, Organic Chemistry, Biomaterials, Biophysics|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry|
|Depositing user:||Mr Derek Boyle|
|Date Deposited:||05 May 2006|
|Last modified:||05 Aug 2016 02:01|