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Detection and identification of labeled DNA by surface enhanced resonance Raman scattering

Graham, D. and Mallinder, B.J. and Smith, W.E. (2000) Detection and identification of labeled DNA by surface enhanced resonance Raman scattering. Biopolymers, 57 (2). pp. 85-91. ISSN 0006-3525

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Abstract

The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 x 10(-12)M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis.