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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Adaptive optics in confocal and two-photon microscopy of rat brain: a single correction per optical section

Girkin, J.M. and Vijverberg, J. and Orazio, M.A. and Poland, S.P. and Wright, A. and Cobb, S. (2007) Adaptive optics in confocal and two-photon microscopy of rat brain: a single correction per optical section. Proceedings of SPIE: The International Society for Optical Engineering, 6442. 64420T.

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Abstract

Sample induced optical aberrations in slices of rat brain tissue have been corrected with a deformable membrane mirror. The aberration correction required by the DMM was determined using a genetic algorithm with the intensity at a point in the sample as a fitness value. We show that by optimising on the intensity of a single point in the sample we are able to improve the axial resolution across the whole field of view of the image at a fixed sample depth. The ratio between the corrected axial resolution and the diffraction limited resolution is on average 2.7 for a 50 µm thick rat brain tissue sample and 12 for a 380 µm thick sample across the whole field of view. The uncorrected ratio being 4.1 and 15.5 respectively. Using a single aberration correction per depth, compared to a point-by-point aberration correction, will significantly decrease scan times and therefore reduce photobleaching and phototoxic effects enabling more rapid microscopy with active aberration correction.