Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Adaptive optics in confocal and two-photon microscopy of rat brain: a single correction per optical section

Girkin, J.M. and Vijverberg, J. and Orazio, M.A. and Poland, S.P. and Wright, A. and Cobb, S. (2007) Adaptive optics in confocal and two-photon microscopy of rat brain: a single correction per optical section. Proceedings of SPIE: The International Society for Optical Engineering, 6442. 64420T. ISSN 0277-786X

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Sample induced optical aberrations in slices of rat brain tissue have been corrected with a deformable membrane mirror. The aberration correction required by the DMM was determined using a genetic algorithm with the intensity at a point in the sample as a fitness value. We show that by optimising on the intensity of a single point in the sample we are able to improve the axial resolution across the whole field of view of the image at a fixed sample depth. The ratio between the corrected axial resolution and the diffraction limited resolution is on average 2.7 for a 50 µm thick rat brain tissue sample and 12 for a 380 µm thick sample across the whole field of view. The uncorrected ratio being 4.1 and 15.5 respectively. Using a single aberration correction per depth, compared to a point-by-point aberration correction, will significantly decrease scan times and therefore reduce photobleaching and phototoxic effects enabling more rapid microscopy with active aberration correction.

Item type: Article
ID code: 9076
Keywords: confocal microscopy, multiphoton microscopy, adaptive optics, aberration correction, spherical-aberration, mirror, implementation, system, water, Optics. Light, Condensed Matter Physics, Electronic, Optical and Magnetic Materials, Electrical and Electronic Engineering, Applied Mathematics, Computer Science Applications
Subjects: Science > Physics > Optics. Light
Department: Faculty of Science > Institute of Photonics
Faculty of Science > Physics
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 03 Nov 2009 09:59
    Last modified: 08 Jul 2014 05:01
    URI: http://strathprints.strath.ac.uk/id/eprint/9076

    Actions (login required)

    View Item