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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy

Wright, A. and Poland, S.P. and Vijverberg, J. and Girkin, J.M. (2007) A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy. In: Adaptive Optics For Industry And Medicine: Proceedings of the Sixth International Workshop. Imperial College Press, London, United Kingdom, pp. 382-387. ISBN 9781848161108

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Abstract

We report on a practical method for the implementation of Adaptive Optics in optical sectioning microscopy to remove system and sample induced aberrations. Correcting for induced aberrations on a pixel-by-pixel basis would take in excess of 4 minutes and greatly increase the risk of sample damage due to photo-bleaching and photo-toxicity; this is clearly an impractical approach. We show that a single aberration correction per optical slice is adequate to significantly improve the image quality across the whole field of view. We present results illustrating the success of this method for a sample scanning and beam scanning system using confocal and multiphoton microscopy.