Picture of person typing on laptop with programming code visible on the laptop screen

World class computing and information science research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

Explore

A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy

Wright, A. and Poland, S.P. and Vijverberg, J. and Girkin, J.M. (2007) A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy. In: Adaptive Optics For Industry And Medicine: Proceedings of the Sixth International Workshop. Imperial College Press, London, United Kingdom, pp. 382-387. ISBN 9781848161108

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

We report on a practical method for the implementation of Adaptive Optics in optical sectioning microscopy to remove system and sample induced aberrations. Correcting for induced aberrations on a pixel-by-pixel basis would take in excess of 4 minutes and greatly increase the risk of sample damage due to photo-bleaching and photo-toxicity; this is clearly an impractical approach. We show that a single aberration correction per optical slice is adequate to significantly improve the image quality across the whole field of view. We present results illustrating the success of this method for a sample scanning and beam scanning system using confocal and multiphoton microscopy.