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Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy

Poland, S.P. and Burns, D. and Lubeigt, W. and Patterson, B.A. and Valentine, G.J. and Wright, A.J. and Girkin, J.M. (2005) Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy. In: 5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings. SPIE. ISBN 9780819460424

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Abstract

Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.