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Assessment of a microscopic photobleaching technique for measuring the spectral absorption efficiency of individual phytoplankton cells

Neumüller, M. and Cunningham, A. and McKee, D.J.C. (2002) Assessment of a microscopic photobleaching technique for measuring the spectral absorption efficiency of individual phytoplankton cells. Journal of Plankton Research, 24 (8). pp. 741-746. ISSN 0142-7873

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Abstract

Measurements of the absorptance of photosynthetic pigments in individual phytoplankton cells were made using an epifluorescence microscope equipped with a spectrograph and CCD array detector. Correction for light loss due to scattering was achieved by bleaching the cells with intense light from a mercury arc lamp, and using the bleached cells as a spectrophotometric blank. Absorption efficiency factors were calculated from knowledge of the geometrical cross-section of the cells obtained fromcalibrated video images acquired at the time of measurement. The single-cell efficiency factors were consistent with the average absorption efficiencies of cell suspensions measured using a spectrophotometer over most of the visible spectrum, but they were significantly lower below 420 nm. Cells of the diatom Cyclotella cryptica and the chlorophyte Chlorella salina showed clear spectral differences in spectral shape that could be related to taxonomic differences in pigment content, but absorption efficiency factors of approximately 0.4 at 675 nm were found for both species.

Item type: Article
ID code: 8619
Keywords: microscopic photobleaching, spectral absorption, phytoplankton cells, physics, plankton research, plankton, Chemistry, Physics, Ecology, Ecology, Evolution, Behavior and Systematics, Aquatic Science
Subjects: Science > Chemistry
Science > Physics
Department: Faculty of Science > Physics
Unknown Department
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 21 Oct 2009 10:26
    Last modified: 04 Sep 2014 19:09
    URI: http://strathprints.strath.ac.uk/id/eprint/8619

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