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Chromium vI induced cytoskeletal damage and cell death in isolated hepatocytes

Gunaratnam, M. and Grant, M.H. (2002) Chromium vI induced cytoskeletal damage and cell death in isolated hepatocytes. In: Biochemical Society Transactions. Biochemical Society, pp. 748-750.

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    Abstract

    Cr(VI) is a known human carcinogen. Although it has been investigated widely, the mechanism(s) of its action is/are not fully understood. The aim of this study was to evaluate Cr(VI)-induced damage to the cell cytoskeleton and the mode of cell death in primary cultures of hepatocytes. Exposure of the cultured cells (10(5)/cm(2)) to 1 and 5 microM Cr(VI) for 24 h resulted in loss of the cell cytoskeleton, and this was accompanied by membrane blebbing and shrinking of the cell. Staining of the cells with annexin V and propidium iodide showed that Cr(VI) induces apoptosis at low concentrations (5 microM), whereas at higher concentrations (25 microM) it induces necrosis. This study shows that Cr(VI) causes damage to the cell cytoskeleton, and induces apoptosis at low concentrations. However, the importance of necrosis and apoptosis in vivo, and the effects of longer exposure times, which simulate environmental and occupational exposure to Cr(VI), remain to be investigated.

    Item type: Book Section
    ID code: 8431
    Keywords: chromium vI, cytoskeletal, cells, hepatocytes, bioengineering, biochemistry, Bioengineering
    Subjects: Technology > Engineering (General). Civil engineering (General) > Bioengineering
    Department: Faculty of Engineering > Bioengineering
    Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 11 Aug 2009 09:44
    Last modified: 06 Sep 2014 21:32
    URI: http://strathprints.strath.ac.uk/id/eprint/8431

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