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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Flying spot en face OCT for monitoring cell distribution in collagen-based constructs

Kosmidis, K. and Russell, Christopher D. and Dobre, George and Podoleanu, Adrian Gh. and Black, R.A. (2007) Flying spot en face OCT for monitoring cell distribution in collagen-based constructs. Multiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE), 6079. 60790Q-1. ISSN 1605-7422

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Abstract

The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.