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The role of endothelial cell attachment to elastic fibre molecules in the enhancement of monolayer formation and retention, and the inhibition of smooth muscle cell recruitment

Williamson, M.R. and Shuttleworth, C.A. and Canfield, A.E. and Black, R.A. and Kielty, C.M. (2007) The role of endothelial cell attachment to elastic fibre molecules in the enhancement of monolayer formation and retention, and the inhibition of smooth muscle cell recruitment. Biomaterials, 28 (35). pp. 5307-5318. ISSN 0142-9612

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Abstract

The endothelium is an essential modulator of vascular tone and thrombogenicity and a critical barrier between the vessel wall and blood components. In tissue-engineered small-diameter vascular constructs, endothelial cell detachment in flow can lead to thrombosis and graft failure. The subendothelial extracellular matrix provides stable endothelial cell anchorage through interactions with cell surface receptors, and influences the proliferation, migration, and survival of both endothelial cells and smooth muscle cells. We have tested the hypothesis that these desired physiological characteristics can be conferred by surface coatings of natural vascular matrix components, focusing on the elastic fiber molecules, fibrillin-1, fibulin-5 and tropoelastin. On fibrillin-1 or fibulin-5-coated surfaces, endothelial cells exhibited strong integrin-mediated attachment in static conditions (82% and 76% attachment, respectively) and flow conditions (67% and 78% cell retention on fibrillin-1 or fibulin-5, respectively, at 25 dynes/cm2), confluent monolayer formation, and stable functional characteristics. Adhesion to these two molecules also strongly inhibited smooth muscle cell migration to the endothelial monolayer. In contrast, on elastin, endothelial cells attached poorly, did not spread, and had markedly impaired functional properties. Thus, fibrillin-1 and fibulin-5, but not elastin, can be exploited to enhance endothelial stability, and to inhibit SMC migration within vascular graft scaffolds. These findings have important implications for the design of vascular graft scaffolds, the clinical performance of which may be enhanced by exploiting natural cell-matrix biology to regulate cell attachment and function.

Item type: Article
ID code: 8290
Keywords: endothelial cells, extracellular matrix, shear stress, adhesion molecules, smooth muscle cells, bioengineering, Bioengineering, Physiology
Subjects: Technology > Engineering (General). Civil engineering (General) > Bioengineering
Science > Physiology
Department: Faculty of Engineering > Bioengineering
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 04 Aug 2009 15:57
    Last modified: 04 Oct 2012 12:37
    URI: http://strathprints.strath.ac.uk/id/eprint/8290

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