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World class computing and information science research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

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Determination of factors affecting the photoreactivation of listeria monocytogenes following exposure to pulsed UV-rich light

Lani, M.N. and Anderson, J.G. and MacGregor, S.J. (2005) Determination of factors affecting the photoreactivation of listeria monocytogenes following exposure to pulsed UV-rich light. In: SGM Meeting Abstracts, 2005-09-12 - 2005-09-14.

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Abstract

Photorepair (photoreactivation) of micro-organisms is an important issue when UV light is used for disinfection purposes. Photoreactivation is a DNA repair process that takes place when cells that have been exposed to, and damaged by, UV light are subsequently exposed to light of a longer wavelength. Although this process has been examined in a number of micro-organisms after their exposure to continuous UV light there is no information available in the literature concerning the effect neither of pulsed UV light nor on the ability of L. monocytogenes to exhibit photorepair after UV light damage. In order to address these issues four factors were compared in this study on L. monocytogenes these being; (i) the effect of the initial dose of pulsed UV light (4 and 6 pulses); (ii) the effect of post exposure conditions on repair/recovery (light or/and dark repair); (iii) the effect of light intensity during photorepair (high intensity versus low intensity light); and (iv) the influence of growth phase (exponential and stationary phase) prior to treatment. The results demonstrate that L. monocytogenes exhibits light repair if the organism is exposed to longer wavelength light following the initial damaging exposure to pulsed high intensity UV-rich light.