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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Influence of cultivation for growth of listeria monocytogenes under different temperature conditions

Lani, M.N. and Anderson, J.G. and MacGregor, S.J. (2004) Influence of cultivation for growth of listeria monocytogenes under different temperature conditions. In: Society for General Microbiology, 155th Meeting, 2004-09-06 - 2004-09-09.

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Abstract

This study compared the performance of various methods for cultivating Listeria monocytogenes in broth medium (Tryptone soya broth with 0.6 % yeast extract). The cultivation methods compared were shaken flask, static flask and a 2.5 litre stirred bioreactor system. Strains NCTC 10357 (serotype 1a) and NCTC 11994 (serotype 4b) were investigated using shaken and static flasks at 37 °C using both the spiral plate method and the conventional plate method for growth assessment. Results demonstrated that the spiral plate enumeration method produced more consistent results and smoother growth curves than the conventional dilution plate method.