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Niosomes and polymeric chitosan based vesicles bearing transferrin and glucose ligands for drug targeting

Dufès, Christine and Schätzlein, A.G. and Tetley, L. and Gray, A.I. and Watson, D.G. and Olivier, J.C. and Couet, W. and Uchegbu, I.F. (2000) Niosomes and polymeric chitosan based vesicles bearing transferrin and glucose ligands for drug targeting. Pharmaceutical Research, 17 (10). pp. 1250-1258. ISSN 0724-8741

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    Abstract

    PURPOSE: To prepare polymeric vesicles and niosomes bearing glucose or transferrin ligands for drug targeting. METHODS: A glucose-palmitoyl glycol chitosan (PGC) conjugate was synthesised and glucose-PGC polymeric vesicles prepared by sonication of glucose-PGC/cholesterol. N-palmitoylglucosamine (NPG) was synthesised and NPG niosomes also prepared by sonication of NPG/ sorbitan monostearate/ cholesterol/ cholesteryl poly-24-oxyethylene ether. These 2 glucose vesicles were incubated with colloidal concanavalin A gold (Con-A gold), washed and visualised by transmission electron microscopy (TEM). Transferrin was also conjugated to the surface of PGC vesicles and the uptake of these vesicles investigated in the A431 cell line (over expressing the transferrin receptor) by fluorescent activated cell sorter analysis. RESULTS: TEM imaging confirmed the presence of glucose units on the surface of PGC polymeric vesicles and NPG niosomes. Transferrin was coupled to PGC vesicles at a level of 0.60+/-0.18 g of transferrin per g polymer. The proportion of FITC-dextran positive A431 cells was 42% (FITC-dextran solution), 74% (plain vesicles) and 90% (transferrin vesicles). CONCLUSIONS: Glucose and transferrin bearing chitosan based vesicles and glucose niosomes have been prepared. Glucose bearing vesicles bind Con-A to their surface. Chitosan based vesicles are taken up by A431 cells and transferrin enhances this uptake.

    Item type: Article
    ID code: 7829
    Keywords: polymeric vesicles, glucose vesicles, transferrin vesicles, Pharmacy and materia medica, Organic Chemistry, Pharmaceutical Science, Molecular Medicine, Biotechnology, Pharmacology, Pharmacology (medical)
    Subjects: Medicine > Pharmacy and materia medica
    Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
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      Depositing user: Strathprints Administrator
      Date Deposited: 03 Apr 2009 13:32
      Last modified: 05 Sep 2014 03:16
      URI: http://strathprints.strath.ac.uk/id/eprint/7829

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