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DNA sequence recognition by an isopropyl substituted thiazole polyamide

James, P.L. and Merkina, E.E. and Khalaf, A.I. and Suckling, C.J. and Waigh, R.D. and Brown, T. and Fox, K.R. (2004) DNA sequence recognition by an isopropyl substituted thiazole polyamide. Nucleic Acids Research, 32 (11). pp. 3410-3417. ISSN 0305-1048

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We have used DNA footprinting and fluorescence melting experiments to study the sequence-specific binding of a novel minor groove binding ligand (thiazotropsin A), containing an isopropyl substituted thiazole polyamide, to DNA. In one fragment, which contains every tetranucleotide sequence, sub-micromolar concentrations of the ligand generate a single footprint at the sequence ACTAGT. This sequence preference is confirmed in melting experiments with fluorescently labelled oligonucleotides. Experiments with DNA fragments that contain variants of this sequence suggest that the ligand also binds, with slightly lower affinity, to sequences of the type XCYRGZ, where X is any base except C, and Z is any base except G.

Item type: Article
ID code: 778
Keywords: DNA sequence, isopropyl substituted thiazole polyamide, fluorescence melting, groove binding ligand, tetranucleotide sequence, fluorescently labelled oligonucleotides, applied chemistry, Chemistry, Genetics
Subjects: Science > Chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Allison Crawford
Date Deposited: 18 Apr 2006
Last modified: 20 Oct 2015 11:31

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