James, P.L. and Merkina, E.E. and Khalaf, A.I. and Suckling, C.J. and Waigh, R.D. and Brown, T. and Fox, K.R. (2004) DNA sequence recognition by an isopropyl substituted thiazole polyamide. Nucleic Acids Research, 32 (11). pp. 3410-3417. ISSN 0305-1048
Full text not available in this repository. (Request a copy from the Strathclyde author)Abstract
We have used DNA footprinting and fluorescence melting experiments to study the sequence-specific binding of a novel minor groove binding ligand (thiazotropsin A), containing an isopropyl substituted thiazole polyamide, to DNA. In one fragment, which contains every tetranucleotide sequence, sub-micromolar concentrations of the ligand generate a single footprint at the sequence ACTAGT. This sequence preference is confirmed in melting experiments with fluorescently labelled oligonucleotides. Experiments with DNA fragments that contain variants of this sequence suggest that the ligand also binds, with slightly lower affinity, to sequences of the type XCYRGZ, where X is any base except C, and Z is any base except G.
| Item type: | Article |
|---|---|
| ID code: | 778 |
| Keywords: | DNA sequence, isopropyl substituted thiazole polyamide, fluorescence melting, groove binding ligand, tetranucleotide sequence, fluorescently labelled oligonucleotides, applied chemistry, Chemistry |
| Subjects: | Science > Chemistry |
| Department: | Faculty of Science > Pure and Applied Chemistry Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences |
| Related URLs: | |
| Depositing user: | Allison Crawford |
| Date Deposited: | 18 Apr 2006 |
| Last modified: | 12 Mar 2012 10:36 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/778 |
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