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Microfluidic DNA amplification - a review

Zhang, Yonghao and Ozdemir, Pinar (2009) Microfluidic DNA amplification - a review. Analytica Chimica Acta, 638 (2). pp. 115-125. ISSN 0003-2670

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    The application of microfluidic devices for DNA amplification has recently been extensively studied. Here, we review the important development of microfluidic polymerase chain reaction (PCR) devices and discuss the underlying physical principles for the optimal design and operation of the device. In particular, we focus on continuous-flow microfluidic PCR on-chip, which can be readily implemented as an integrated function of a micro-total-analysis system. To overcome sample carryover contamination and surface adsorption associated with microfluidic PCR, microdroplet technology has recently been utilized to perform PCR in droplets, which can eliminate the synthesis of short chimeric products, shorten thermal-cycling time, and offers great potential for single DNA molecule and single-cell amplification. The work on chip-based PCR in droplets is highlighted.

    Item type: Article
    ID code: 7763
    Notes: IF-3.186, HL-6.2
    Keywords: PCR, microfluidics, DNA amplification, microdroplet technology, Lab-on-a-chip, μTAS, Mechanical engineering and machinery, Physical and theoretical chemistry, Mechanical Engineering, Fluid Flow and Transfer Processes
    Subjects: Technology > Mechanical engineering and machinery
    Science > Chemistry > Physical and theoretical chemistry
    Department: Faculty of Engineering > Mechanical and Aerospace Engineering
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      Depositing user: Strathprints Administrator
      Date Deposited: 25 Mar 2009 14:24
      Last modified: 08 Feb 2015 05:29

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