Picture of virus under microscope

Research under the microscope...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

Explore SIPBS research

Na+/Ca2+ exchanger expression and function in a rabbit model of myocardial infarction

Smith, G.L. and Elliot, E.E.B. and Kettlewell, S. and Currie, S. and Quinn, F.R. (2006) Na+/Ca2+ exchanger expression and function in a rabbit model of myocardial infarction. Journal of Cardiovascular Electrophysiology, 17 (1). S57-S63. ISSN 1045-3873

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

In general, sarcolemmal Na+/Ca2+ exchanger (NCX) protein and activity is increased in hearts with ventricular dysfunction. However, in a subset of studies, reduced activity of NCX has been reported. Left ventricular dysfunction (LVD) was induced in the rabbit eight weeks after an apical myocardial infarction. Using single microelectrode voltage clamp to assess the NCX activity in isolated ventricular cells, a decrease in NCX activity by ∼30% was observed. Immunoblot analysis indicated increased NCX protein levels by ∼20% in the LVD group. The cause of this paradox is unknown. Overexpression of the protein sorcin increased the activity of NCX without affecting NCX protein levels. Sorcin protein (dimer) levels were significantly lower in the LVD group (0.67 ± 0.05 n = 15, P < 0.05) compared to sham (1.0 ± 0.16, n = 15). Sorcin monomer levels were not significantly different (sham: 1.0 ± 0.26, LVD: 0.83 ± 0.13). Mathematical modeling of NCX suggests that a reduction of NCX activity during diastole to that in LVD could be achieved by holding the diastolic membrane potential at −60 mV instead of −80 mV. Holding Em at −60 mV decreased NCX-mediated Ca2+ efflux rates to values comparable to those seen in LVD and increased SR Ca2+ content and peak systolic [Ca2+] in sham and LVD cardiomyocytes. In conclusion, reduced sorcin expression may be linked to the lower NCX activity in the rabbit model of LVD. Reduced NCX activity during diastole increases SR Ca2+ content and Ca2+ transient amplitude.