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The potential of mid infrared spectroscopy (MIRS) for real time bioprocess monitoring

McNeil, B. and Harvey, L.M. and Roychoudhury, P. (2006) The potential of mid infrared spectroscopy (MIRS) for real time bioprocess monitoring. Analytica Chimica Acta, 571 (2). pp. 159-166. ISSN 0003-2670

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Abstract

The need for effective bioprocess (fermentation) monitoring is growing in importance due to the rapid pace of change in the fermentation industry, and attendant financial pressures. Vibrational spectroscopy has shown great promise in bioprocess monitoring. In particular, recently attention has been focused on the capability of mid infrared spectroscopy (MIRS) to monitor multiple analytes in highly complex fermentation fluids. The potential of this powerful analytical technique is critically evaluated by discussion of relevant studies. The advantages and limitations of MIR are discussed in the context of "rival" technologies, such as near infrared, focusing especially on employing such techniques in bioprocesses for real time (either in situ or ex situ) measurements. The potential barriers to the development of MIRS for real time monitoring are identified and further research directions highlighted.