McNeil, B. and Finn, B. and Harvey, L.M. (2006) Near infrared spectroscopic monitoring biomass, glucose, ethanol and protein content in a high cell density baker's yeast fed-batch culture. Yeast, 23 (7). pp. 507-517. ISSN 0749-503XFull text not available in this repository. (Request a copy from the Strathclyde author)
The use of at-line NIRS to monitor a high cell density fed-batch baker's yeast bioprocess was investigated. Quantification of the key analytes (biomass, ethanol and glucose) and the product quality indicator (percentage protein content) was studied. Biomass was quantitatively modelled using whole matrix samples (as was percentage protein content). The dominance of the whole matrix spectrum by biomass, and its associated light scattering effects, were overcome by use of filtrate samples and adapted (semi-synthetic) filtrate samples, which allowed successful ethanol and glucose modelling, respectively. Calibrations were rigorously challenged via external validation with large sample sets relative to the calibration sample size, ensuring model robustness and potential practical utility. The standard errors of calibration for biomass, glucose, ethanol and total intracellular protein were (g/l) 1.79, 0.19, 0.79 and 0.91, respectively, comparable to those of the primary assays. The calibration strategies necessary to generate quantitative models for this range of analytes in such a complex high cell density bioprocess fluid are discussed.
|Keywords:||near-infrared spectroscopy, saccharomyces cerevisiae, bioprocess monitoring, high cell density, fed-batch bioprocess, pharmacology, biomedical sciences, Pharmacy and materia medica, Biochemistry, Applied Microbiology and Biotechnology, Genetics, Bioengineering, Biotechnology|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
|Depositing user:||Strathprints Administrator|
|Date Deposited:||14 Apr 2009 10:57|
|Last modified:||21 Apr 2017 04:27|