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Near infrared spectroscopic monitoring biomass, glucose, ethanol and protein content in a high cell density baker's yeast fed-batch culture

McNeil, B. and Finn, B. and Harvey, L.M. (2006) Near infrared spectroscopic monitoring biomass, glucose, ethanol and protein content in a high cell density baker's yeast fed-batch culture. Yeast, 23 (7). pp. 507-517. ISSN 0749-503X

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Abstract

The use of at-line NIRS to monitor a high cell density fed-batch baker's yeast bioprocess was investigated. Quantification of the key analytes (biomass, ethanol and glucose) and the product quality indicator (percentage protein content) was studied. Biomass was quantitatively modelled using whole matrix samples (as was percentage protein content). The dominance of the whole matrix spectrum by biomass, and its associated light scattering effects, were overcome by use of filtrate samples and adapted (semi-synthetic) filtrate samples, which allowed successful ethanol and glucose modelling, respectively. Calibrations were rigorously challenged via external validation with large sample sets relative to the calibration sample size, ensuring model robustness and potential practical utility. The standard errors of calibration for biomass, glucose, ethanol and total intracellular protein were (g/l) 1.79, 0.19, 0.79 and 0.91, respectively, comparable to those of the primary assays. The calibration strategies necessary to generate quantitative models for this range of analytes in such a complex high cell density bioprocess fluid are discussed.

Item type: Article
ID code: 7553
Keywords: near-infrared spectroscopy, saccharomyces cerevisiae, bioprocess monitoring, high cell density, fed-batch bioprocess, pharmacology, biomedical sciences, Pharmacy and materia medica, Biochemistry, Applied Microbiology and Biotechnology, Genetics, Bioengineering, Biotechnology
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Unknown Department
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 14 Apr 2009 11:57
    Last modified: 04 Sep 2014 17:12
    URI: http://strathprints.strath.ac.uk/id/eprint/7553

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