Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

An antibody to the aggregated synthetic prion protein peptide (PrP106-126) selectively recognizes disease-associated prion protein (PrPSc) from human brain specimens

Jones, Michael and Wight, Darren and McLoughlin, Victoria and Norrby, Katherine and Ironside, James W. and Connolly, John G. and Farquhar, Christine F. and MacGregor, Ian R. and Head, Mark W. (2009) An antibody to the aggregated synthetic prion protein peptide (PrP106-126) selectively recognizes disease-associated prion protein (PrPSc) from human brain specimens. Brain Pathology, 19 (2). pp. 293-302. ISSN 1015-6305

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Human prion diseases are characterized by the conversion of the normal host cellular prion protein (PrPC) into an abnormal misfolded form [disease-associated prion protein (PrPSc)]. Antibodies that are capable of distinguishing between PrPC and PrPSc may prove to be useful, not only for the diagnosis of these diseases, but also for a better understanding of the molecular mechanisms involved in disease pathogenesis. In an attempt to produce such antibodies, we immunized mice with an aggregated peptide spanning amino acid residues 106 to 126 of human PrP (PrP106-126). We were able to isolate and single cell clone a hybridoma cell line (P1:1) which secreted an IgM isotype antibody [monoclonal antibody (mAb P1:1)] that recognized the aggregated, but not the monomeric form of the immunogen. When used in immunoprecipitation assays, the antibody did not recognize normal PrPC from non-prion disease brain specimens, but did selectively immunoprecipitate full-length PrPSc from cases of variant and sporadic Creutzfeldt-Jakob disease and Gerstmann-Straussler-Scheinker disease. These results suggest that P1:1 recognizes an epitope formed during the structural rearrangement or aggregation of the PrP that is common to the major PrPSc types found in the most common forms of human prion disease.

Item type: Article
ID code: 7534
Keywords: creutzfeldt-jakob disease, immunoprecipitation, monoclonal antibody, prion protein, PrP106–126, Pharmacy and materia medica, Clinical Neurology, Neuroscience(all), Pathology and Forensic Medicine
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 12 Feb 2009 16:52
    Last modified: 04 Sep 2014 18:39
    URI: http://strathprints.strath.ac.uk/id/eprint/7534

    Actions (login required)

    View Item