Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

High-performance liquid chromatography determination of σ1-acid glycoprotein in small volumes of plasma from neonates

Stumpe, M. and Miller, C. and Morton, N.S. and Bell, G. and Watson, D.G. (2006) High-performance liquid chromatography determination of σ1-acid glycoprotein in small volumes of plasma from neonates. Journal of Chromatography B, 831 (1-2). pp. 81-84. ISSN 1570-0232

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

In order to investigate how the α1-acid glycoprotein (AGP) concentrations of neonates change in response to surgical stress, a simple high-performance liquid chromatography (HPLC)-assay for the measurement of α1-acid glycoprotein levels was developed. A fraction containing α1-acid glycoprotein was isolated from the bulk of plasma protein by addition of 0.6 M perchloric acid and was then analysed directly on a short PLRP-S 4000 Å reversed phase column column. The method was validated by analysis of pooled plasma from healthy adults both in comparison with a calibration curve and by standard additions. The procedure was able to isolate α1-acid glycoprotein rapidly (<30 min) and required only 50 μl of plasma. The mean extraction recovery was 79.1% (CV 6.4%). The within-run precision for the analysis of three replicates of quality control sample ranged from ±1.2 to ±3.8% and the between-run precision was ±6.1%. The method was linear (r2 = 0.988) over a concentration range from 6 to 100.0 mg/100 ml. The AGP levels in neonatal samples ranged from 25 to 93 mg/100 ml.

Item type: Article
ID code: 7529
Keywords: anaesthetics, alpha1-acid glycoprotein, HPLC, neonates, plasma, chromatography, Therapeutics. Pharmacology, Biochemistry, Cell Biology, Analytical Chemistry, Clinical Biochemistry
Subjects: Medicine > Therapeutics. Pharmacology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences > Pharmaceutical Sciences
Unknown Department
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 22 Feb 2009 15:15
    Last modified: 04 Sep 2014 17:21
    URI: http://strathprints.strath.ac.uk/id/eprint/7529

    Actions (login required)

    View Item