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The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

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The cytotoxicity of chromium in osteoblasts: effects on macromolecular synthesis

Denning, D.J. and Henderson, C.J. and Grant, M.H. (2002) The cytotoxicity of chromium in osteoblasts: effects on macromolecular synthesis. Journal of Materials Science: Materials in Medicine, 13 (1). pp. 47-52. ISSN 0957-4530

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Abstract

Exposure of an immortalized rat osteoblast cell line, FFC cells, to Cr VI resulted in inhibition of protein, DNA and RNA synthesis. Protein synthesis (^3\hbox{H}-leucine incorporation) was most sensitive. There was no inhibition of the incorporation of ^{3}\hbox{H}-proline into collagen at the concentrations which inhibited general protein synthesis (1 muM), but synthesis of extracellular collagen fibers was markedly decreased by concentrations of 0.5 muM Cr VI and above. This indicates that some aspect of the post-translational processing of the collagen fibers is sensitive to Cr VI inhibition. Collagen fiber formation was not inhibited by Cr III (which does not penetrate the cell membrane) or when Cr VI was reduced to Cr III extracellularly. This suggests that the Cr VI inhibits an intracellular stage of post-translational collagen processing. Both Cr VI and Cr III inhibit collagenase activity, the former being more potent but less efficacious. Our results suggest that leakage of chromium ions from orthopedic implants may cause a decrease in the proliferation and infiltration of osteoblasts around the implant, and a reduction in the synthesis and altered turnover of collagen in extracellular matrix. These effects will influence the osseointegration of implants, the osteolytic response, and ultimately the stable life-time of the implants.