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The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the Physical Activity for Health Group based within the School of Psychological Sciences & Health. Research here seeks to better understand how and why physical activity improves health, gain a better understanding of the amount, intensity, and type of physical activity needed for health benefits, and evaluate the effect of interventions to promote physical activity.

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Expression of glutathione-S-transferases in hepatocytes cultured on collagen gels of different composition

Kataropoulou, M. and Henderson, C.J. and Grant, M.H. (2002) Expression of glutathione-S-transferases in hepatocytes cultured on collagen gels of different composition. Toxicology, 192. pp. 87-88. ISSN 0300-483X

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Abstract

The use of primary hepatocyte cultures as in vitro models for studying xenobiotic metabolism and toxicity is limited by the loss of liver-specific differentiated functions with time in culture. This study investigates the effect of incorporating 20% chondroitin-6-sulphate (Ch6SO4), a glycosaminoglycan (GAG), into collagen gels (0.3% w/v), and crosslinking the gels with either 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) or 1,6-diaminohexane (DAH) on the expression and total activity of glutathione-S-transferases (GSTs) in hepatocytes cultured for 7 days. GSTs are a family of isoenzymes. The main isoenzyme in the male rat liver is the alpha form (which exists as several subunits (Ya and Yc2 were measured here)). The pi form (subunit Yf) is not normally present in liver, but is expressed in conditions where the liver cells are de-differentiated.