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Expression of glutathione-S-transferases in hepatocytes cultured on collagen gels of different composition

Kataropoulou, M. and Henderson, C.J. and Grant, M.H. (2002) Expression of glutathione-S-transferases in hepatocytes cultured on collagen gels of different composition. Toxicology, 192. pp. 87-88. ISSN 0300-483X

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Abstract

The use of primary hepatocyte cultures as in vitro models for studying xenobiotic metabolism and toxicity is limited by the loss of liver-specific differentiated functions with time in culture. This study investigates the effect of incorporating 20% chondroitin-6-sulphate (Ch6SO4), a glycosaminoglycan (GAG), into collagen gels (0.3% w/v), and crosslinking the gels with either 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) or 1,6-diaminohexane (DAH) on the expression and total activity of glutathione-S-transferases (GSTs) in hepatocytes cultured for 7 days. GSTs are a family of isoenzymes. The main isoenzyme in the male rat liver is the alpha form (which exists as several subunits (Ya and Yc2 were measured here)). The pi form (subunit Yf) is not normally present in liver, but is expressed in conditions where the liver cells are de-differentiated.

Item type: Article
ID code: 6779
Notes: Selected Papers and Abstracts of the British Toxicology Society Annual Congress
Keywords: liver, toxicology, bioengineering, collagen, hepatocytes, Bioengineering, Medicine (General), Human anatomy, Toxicology
Subjects: Technology > Engineering (General). Civil engineering (General) > Bioengineering
Medicine > Medicine (General)
Science > Human anatomy
Department: Faculty of Engineering > Bioengineering
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 28 Aug 2008
    Last modified: 04 Sep 2014 14:57
    URI: http://strathprints.strath.ac.uk/id/eprint/6779

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