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Anti-GQ1b ganglioside antibodies mediate complement-dependent destruction of the motor nerve terminal

O'Hanlon, G.M. and Plomp, J.J. and Chakrabarti, M. and Morrison, I. and Wagner, E.R. and Goodyear, C.S. and Yin, X. and Trapp, B.D. and Conner, J. and Molenaar, P.C. and Stewart, S. and Rowan, E.G. and Willison, H.J. (2001) Anti-GQ1b ganglioside antibodies mediate complement-dependent destruction of the motor nerve terminal. Brain, 124 (5). pp. 893-906. ISSN 0006-8950

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Abstract

Miller-Fisher syndrome is an autoimmune neuropathy characterized by ataxia, areflexia and ophthalmoplegia, and in the majority of cases the presence of high titres of anti-GQ1b ganglioside antibodies. In an ex vivo model, human and mouse anti-GQ1b antibodies have been shown previously to induce a complement-dependent {alpha}-latrotoxin-like effect on the murine motor endplate, i.e. they bring about massive quantal release of acetylcholine and eventually block neuromuscular transmission. Using immunofluorescence microscopy with image analysis, we show here that the late stages of this electrophysiological effect temporally coincide with the loss of heavy neurofilament (200 kDa) and type III ß-tubulin immunostaining and structural breakdown of the nerve terminal, as demonstrated by electron microscopy. Ultrastructurally, axon terminals were disorganized, depleted of vesicles, and subdivided by the infiltrating processes of capping Schwann cells. These findings provide clear pathological evidence to support a role for anti-ganglioside antibodies in mediating nerve terminal injury and further advance the view that this site may be of importance as a target in some human neuropathies.

Item type: Article
ID code: 5481
Keywords: brain, biomedical science, neuropathy, ataxia, areflexia, ophthalmoplegia, autoantibody, complement, ganglioside, miller-fisher syndrome, neuromuscular junction, Pharmacy and materia medica, Physiology, Clinical Neurology
Subjects: Medicine > Pharmacy and materia medica
Science > Physiology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Humanities and Social Sciences (HaSS) > School of Social Work and Social Policy > Social Work
Faculty of Humanities and Social Sciences (HaSS) > School of Psychological Science and Health > Counselling
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Depositing user: Strathprints Administrator
Date Deposited: 27 Feb 2008
Last modified: 04 Sep 2014 14:30
URI: http://strathprints.strath.ac.uk/id/eprint/5481

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