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A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro

Baillie, G. and Owens, M. and Halbert, G.W. (2002) A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro. Journal of Lipid Research, 43. pp. 69-73. ISSN 0022-2275

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Abstract

A synthetic LDL (sLDL) has been prepared by combining a lipid microemulsion with amphipathic peptides containing the apoprotein B receptor domain. The biological properties of sLDL have been investigated using the U937 in vitro cell proliferation assay. sLDL exhibits a concentration dependent and saturable stimulation of U937 proliferation. By utilizing different amphipathic peptides, variable proliferation is achieved, indicating a specific interaction between sLDL and the U937 LDL receptor are possible. U937 proliferation is reduced by the addition of an anti-LDL receptor antibody, indicating that sLDL is assimilated via the LDL receptor pathway. The behavior of sLDL mimics that of native LDL, and this approach represents a viable technique for the production of an sLDL particle on a large scale for research and general application.

Item type: Article
ID code: 5441
Keywords: U937, low density lipoprotein, amphipathic peptide, apoB, synthetic LDL, phosphate-buffered saline, dimethyl sulfoxide, Pharmacy and materia medica
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Unknown Department
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 05 Mar 2008
    Last modified: 12 Mar 2012 10:42
    URI: http://strathprints.strath.ac.uk/id/eprint/5441

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