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World class computing and information science research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

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Beyond monochromatic light: three-dimensional confocal laser scanning microscopy using a supercontinuum source

McConnell, G. and Girkin, J.M. and Poland, S. (2006) Beyond monochromatic light: three-dimensional confocal laser scanning microscopy using a supercontinuum source. Proceedings of SPIE: The International Society for Optical Engineering, 6047. 2Q1 - 2Q5.

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Abstract

Confocal laser scanning microscopy (CLSM) has rapidly become an essential tool in the life sciences laboratory, enabling high-resolution and minimally intrusive optical sectioning of fluorescent samples. Most commercially available CLSM systems employ a gas laser, e.g. a Kr/Ar laser, to provide the excitation radiation. However, such lasers have several shortcomings, including the maintenance requirements, short lifetimes and high noise levels. To overcome these limitations, a light source for CLSM that is based on supercontinuum generation in photonic crystal fiber has been developed. This source provides the necessary wavelength range required to excite the widest possible variety of fluorophores. A novel method of extracting the desired wavelengths from the supercontinuum source using a digital micro-mirror device (DMD) is also described.