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Beyond monochromatic light: three-dimensional confocal laser scanning microscopy using a supercontinuum source

McConnell, G. and Girkin, J.M. and Poland, S. (2006) Beyond monochromatic light: three-dimensional confocal laser scanning microscopy using a supercontinuum source. Proceedings of SPIE: The International Society for Optical Engineering, 6047. 2Q1 - 2Q5.

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Confocal laser scanning microscopy (CLSM) has rapidly become an essential tool in the life sciences laboratory, enabling high-resolution and minimally intrusive optical sectioning of fluorescent samples. Most commercially available CLSM systems employ a gas laser, e.g. a Kr/Ar laser, to provide the excitation radiation. However, such lasers have several shortcomings, including the maintenance requirements, short lifetimes and high noise levels. To overcome these limitations, a light source for CLSM that is based on supercontinuum generation in photonic crystal fiber has been developed. This source provides the necessary wavelength range required to excite the widest possible variety of fluorophores. A novel method of extracting the desired wavelengths from the supercontinuum source using a digital micro-mirror device (DMD) is also described.

Item type: Article
ID code: 5300
Notes: Proceedings title: Fourth International Conference on Photonics and Imaging in Biology and Medicine.
Keywords: confocal laser scanning microscopy, supercontinuum generation, digital micro-mirror device, photonic crystal fiber, Optics. Light, Condensed Matter Physics, Electronic, Optical and Magnetic Materials, Electrical and Electronic Engineering, Applied Mathematics, Computer Science Applications
Subjects: Science > Physics > Optics. Light
Department: Faculty of Science > Institute of Photonics
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Strathprints Administrator
Date Deposited: 03 Feb 2008
Last modified: 10 Dec 2015 17:20

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