Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Use of adaptive optics for improved multiphoton imaging

Girkin, J.M. and Marsh, P.N. (2004) Use of adaptive optics for improved multiphoton imaging. Proceedings of SPIE: The International Society for Optical Engineering, 5323. p. 260. ISSN 1605-7422

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

We report on the integration of active optical elements in a multiphoton microscope to improve theresolution and overall image quality when imaging deeply into biological samples. Optical models were generated of sample systems and these have been compared with the performance of thecomplete imaging system. The active elements used were commercially available flexible membranemirrors controlled by custom, home written, software. Significant improvements in image qualityhave been demonstrated using a range of optimisation routines based on the analysis of the imagesproduced by the system, rather than with a wavefront sensor. A three-fold increase in the resolution100µm into the sample was achieved.

Item type: Article
ID code: 5284
Keywords: multiphoton imaging, adaptive optics, microscopy, deep tissue imaging, spherical-aberration, confocal microscopy, 2-photon microscope, compensation, Optics. Light, Condensed Matter Physics, Electronic, Optical and Magnetic Materials, Electrical and Electronic Engineering, Applied Mathematics, Computer Science Applications
Subjects: Science > Physics > Optics. Light
Department: Faculty of Science > Institute of Photonics
Unknown Department
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 01 Feb 2008
    Last modified: 08 Jul 2014 05:00
    URI: http://strathprints.strath.ac.uk/id/eprint/5284

    Actions (login required)

    View Item