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Blue laser diode excited fluroescence spectroscopy of natural caries lesions

Girkin, J.M. and Rousseau, C. and Hall, A.F. and Strang, R. and Whitters, C.J. and Creanor, S.L. and Cribeiro, A. (2004) Blue laser diode excited fluroescence spectroscopy of natural caries lesions. Multiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE), 5313. pp. 1-6. ISSN 1605-7422

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We report on the use of a blue laser diode to excite natural fluorescence from a range of dentalsamples. Sixty teeth with naturally occurring lesions were examined. The lesions were classified onvisual inspection as falling into three classes as shiny lesions, dull lesions and brown lesions. Theresulting fluorescence was then examined using a fibre optically coupled spectrometer in a series ofwavelength bands. By examining the intensity ratios in the two wavelength bands 480-500nm and620 to 640nm we were able to classify the lesion types and thus provide a quantifiable measurementof lesion types. This method has application for detection of dental caries as well as demonstratingpotential application to evaluate lesions which may represent different degrees of caries activity.