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Full system considerations for multiphoton microscopy

Wokosin, D.L. and Girkin, J.M. (2001) Full system considerations for multiphoton microscopy. In: Multiphoton Microscopy in the Biomedical Sciences (Proceedings of SPIE Volume: 4262). SPIE, pp. 231-237.

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Abstract

A most-important-variables analysis of practical, successful multiphoton excitation fluorescence microscopy is presented. The key strength of multiphoton imaging -- localization of the excitation volume -- helps to decouple the excitation from the emission; the emission no longer needs to be imaged. The presentation starts with maximizing the detected signal and proceeds to the laser source considerations. The main goal is to match the instrumentation to the biological problem. The main aspects covered are... Emission collection: potential signal; Focal plane mismatch: chromatic aberration; Light detected: actual signal; Live cell imaging: preservation of biological function; Deep imaging: preservation of image contrast; Signal production: potential limitations.

Item type: Book Section
ID code: 5281
Keywords: optics, photonics, multiphoton microscopy, Optics. Light
Subjects: Science > Physics > Optics. Light
Department: Faculty of Science > Institute of Photonics
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences > Physiology and Pharmacology
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 01 Feb 2008
    Last modified: 17 Jul 2013 13:03
    URI: http://strathprints.strath.ac.uk/id/eprint/5281

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