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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including those from the School of Psychological Sciences & Health - but also papers by researchers based within the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

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Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy

McConnell, G. and Poland, S.P. and Girkin, J.M. (2006) Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy. Review of Scientific Instruments, 77 (013702). 013702. ISSN 0034-6748

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Abstract

We report on the use of a computer-controlled digital micromirror device (DMD) to select discrete wavelength ranges from a white-light supercontinuum and the application of the light source to confocal laser scanning microscopy (CLSM). The fast switching rate of the DMD enabled video-rate wavelength tuning, while the high resolution allowed precise control over the bandwidth selection. The DMD system was used in conjunction with a CLSM system to characterize the wavelength switching capability, prior to performing three-dimensional CLSM of a variety of multiple-labeled samples in order to demonstrate the efficacy and practical application of the system.