Picture of athlete cycling

Open Access research with a real impact on health...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the Physical Activity for Health Group based within the School of Psychological Sciences & Health. Research here seeks to better understand how and why physical activity improves health, gain a better understanding of the amount, intensity, and type of physical activity needed for health benefits, and evaluate the effect of interventions to promote physical activity.

Explore open research content by Physical Activity for Health...

Efficacy of 405nm light for inactivation of feline calicivirus : a surrogate for norovirus

Tomb, Rachael and MacLean, Michelle and Coia, John and MacGregor, Scott and Graham, Libby and MacDonald, Mike and Anderson, John (2014) Efficacy of 405nm light for inactivation of feline calicivirus : a surrogate for norovirus. In: The 9th Healthcare Infection Society International Conference 2014, 2014-11-16 - 2014-11-18, Lyon Convention Centre.

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

Background: Norovirus (NoV) is a leading cause of nosocomial gastroenteritis. With only 10-100 viral particles required for infection, effective decontamination of hospitals is particularly important in preventing the spread of this highly transmissible virus. A technology for continuous environmental decontamination has been developed which utilises 405nm light to inactivate bacteria and fungi, via ROS-generated oxidative damage. Aim(s)/Objectives: To date, the antiviral efficacy of 405nm light has not been fully determined, therefore this study was designed to investigate whether feline calicivirus (FCV), the standard surrogate for NoV, can be inactivated by 405nm light, and how viral susceptibility may be influenced when suspended in biologically-relevant material. Method: FCV was exposed to 405nm light (155.8mW/cm2) whilst suspended in various minimal andnutrient-rich media and inactivation curves were used to establish sensitivity. Viralin activation was measured by assessing infection of a feline embryo cell line (FEA). Results: Antiviral activity of 405nm light against FCV was successfully demonstrated, with a 4-log10(99.99%) reduction in minimal media evident after a dose of 2.8kJ/cm2. However susceptibility was significantly enhanced when exposed in nutrient-rich DMEM and artificial saliva, with an approximate 5-fold reduction in dose required (420J/cm2)for an equivalent level of inactivation. Discussion & Conclusion: The results indicate that FCV can be inactivated using 405nm light, and susceptibility can be significantly enhanced when the virus is present in nutrient-rich, or biologically-relevant material such as saliva. Further research in this area could lead to the development of 405nm light technology for effective NoV decontamination within the hospital environment.