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The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

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Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with Fura-2

McConnell, G. and Riis, E. (2004) Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with Fura-2. Physics in Medicine and Biology, 49 (20). pp. 4757-4763. ISSN 0031-9155

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Abstract

We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.