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Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with Fura-2

McConnell, G. and Riis, E. (2004) Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with Fura-2. Physics in Medicine and Biology, 49 (20). pp. 4757-4763. ISSN 0031-9155

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Abstract

We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.