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Confocal laser scanning fluorescence microscopy with a visible continuum source

McConnell, G. (2004) Confocal laser scanning fluorescence microscopy with a visible continuum source. Optics Express, 12 (13). pp. 2844-2851. ISSN 1094-4087

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Abstract

Confocal laser scanning fluorescence microscopy is demonstrated using a photonic crystal fiber-based excitation source. A 38 cm-long section of photonic crystal fiber is pumped with femtosecond pulses from a Ti:sapphire laser, and the resultant visible continuum is selectively filtered to provide the peak excitation wavelengths required for a range of fluorescently labeled biological tissue.