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Spherical molecularly imprinted polymer particles: A promising tool for molecular recognition in capillary electrokinetic separations

de Boer, T. and Mol, R. and de Zeeuw, R.A. and de Jong, G.J. and Sherrington, D.C. and Cormack, P.A.G. and Ensing, K. (2002) Spherical molecularly imprinted polymer particles: A promising tool for molecular recognition in capillary electrokinetic separations. Electrophoresis, 23 (9). pp. 1296-1300. ISSN 0173-0835

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Abstract

Spherical molecularly imprinted polymer particles obtained via precipitation polymerization, were introduced as a pseudostationary phase in capillary electrophoresis (CE) to study molecular recognition. Analyses were performed via a partial filling technique using (+)-ephedrine-imprinted microspheres (100-200 nm) which were polymerized from methacrylic acid and 1,1,1-Tris(hydroxymethyl)propanetrimethacrylate using acetonitrile as the solvent. The influence of pH and the modifier content on the separation was investigated. A 0.1 % w/v suspension in an aqueous 10 mm phosphate buffer (pH 2.5 with 40% acetonitrile) was hydrodynamically injected into the CE system (80% of the effective capillary length) and led to full baseline separation of racemic ephedrine within 10 min.