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Inactivation of mycobacterium paratuberculosis by pulsed electric fields

Rowan, N.J. and MacGregor, S.J. and Anderson, J.G. and Cameron, Douglas and Farish, O. (2001) Inactivation of mycobacterium paratuberculosis by pulsed electric fields. Applied and Environmental Microbiology, 67 (6). pp. 2833-2836. ISSN 0099-2240

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Abstract

The influence of treatment temperature and pulsed electric fields (PEF) on the viability of Mycobacterium paratuberculosis cells suspended in 0.1% (wt/vol) peptone water and in sterilized cow's milk was assessed by direct viable counts and by transmission electron microscopy (TEM). PEF treatment at 50°C (2,500 pulses at 30 kV/cm) reduced the level of viable M. paratuberculosis cells by approximately 5.3 and 5.9 log10 CFU/ml in 0.1% peptone water and in cow's milk, respectively, while PEF treatment of M. paratuberculosis at lower temperatures resulted in less lethality. Heating alone at 50°C for 25 min or at 72°C for 25 s (extended high-temperature, short-time pasteurization) resulted in reductions of M. paratuberculosis of approximately 0.01 and 2.4 log10 CFU/ml, respectively. TEM studies revealed that exposure to PEF treatment resulted in substantial damage at the cellular level to M. paratuberculosis.