Picture of person typing on laptop with programming code visible on the laptop screen

World class computing and information science research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

Explore

Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

Jeorrett, Abigail H and Neale, Steven L and Massoubre, David and Gu, Erdan and Henderson, Robert K and Millington, Owain and Mathieson, Keith and Dawson, Martin D (2014) Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells. Optics Express, 22 (2). pp. 1372-1380. ISSN 1094-4087

[img]
Preview
PDF (oe-22-2-1372)
oe_22_2_1372.pdf - Final Published Version
License: Creative Commons Attribution 4.0 logo

Download (1MB) | Preview

Abstract

A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.