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Open Access research that challenges the mind...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including those from the School of Psychological Sciences & Health - but also papers by researchers based within the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

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Two-photon laser scanning fluorescence microscopy using photonic crystal fibre

McConnell, G. and Riis, E. (2004) Two-photon laser scanning fluorescence microscopy using photonic crystal fibre. Journal of Biomedical Optics, 9 (5). pp. 922-927. ISSN 1083-3668

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Abstract

We report the application of a simple yet powerful modular pulse compression system, based on photonic crystal fibres which improves upon incumbent twophoton laser scanning fluorescence microscopy techniques. This system provided more than a 7-fold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of infrared radiaton of less than 35 fs duration reaching the sample.