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Two-photon laser scanning fluorescence microscopy using photonic crystal fibre

McConnell, G. and Riis, E. (2004) Two-photon laser scanning fluorescence microscopy using photonic crystal fibre. Journal of Biomedical Optics, 9 (5). pp. 922-927. ISSN 1083-3668

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Abstract

We report the application of a simple yet powerful modular pulse compression system, based on photonic crystal fibres which improves upon incumbent twophoton laser scanning fluorescence microscopy techniques. This system provided more than a 7-fold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of infrared radiaton of less than 35 fs duration reaching the sample.