Li, Chenhao and Sutter, Jens and Birch, David and Chen, Yu (2012) Fluorescence anisotropy of protein - gold nanoclusters. [Proceedings Paper]Full text not available in this repository. (Request a copy from the Strathclyde author)
Red-emitting gold nanoclusters (BSA-Au-25) have been synthesized in Bovine Serum albumin and found to have a characteristic fluorescence lifetime of 1.25 mu s. Fluorescence anisotropy measurements of BSA-Au-25 reveal a dramatic increase in protein size as the pH is reduced from 7 to 3.1, consistent with the change of BSA from N form to E form. This study suggests the possibility of exploiting fluorescent protein-gold nanoclusters to probe protein conformational changes in response to the local environment.
|Item type:||Proceedings Paper|
|Keywords:||gold nanoclusters, fluorescence anisotropy, BSA, protein conformational change , color , proteins , probes, green products , gold, fluorescence, detectors, Physics|
|Subjects:||Science > Physics|
|Department:||Faculty of Science > Physics|
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|Depositing user:||Pure Administrator|
|Date Deposited:||05 Dec 2012 14:42|
|Last modified:||12 Jun 2013 14:35|
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