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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Creating permanent 3D arrangements of isolated cells using holographic optical tweezers

Jordan, P. and Leach, J. and Padgett, M.J. and Blackburn, P. and Isaacs, N. and Goksor, M. and Hanstorp, D. and Wright, A.J. and Girkin, J.M. and Cooper, J. (2005) Creating permanent 3D arrangements of isolated cells using holographic optical tweezers. Lab on a Chip, 5 (11). pp. 1224-1228. ISSN 1473-0197

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Abstract

Reports the creation of permanent 3D configurations of cells, at predefined positions, within a gelatin matrix. The technique used holographic optical tweezers to manipulate individual E. coli within a solution comprising monomer precursors. The matrix was then set and after the laser beam was removed, we were able to demonstrate that the structures remained intact for many days. We were also able to demonstrate that, in the presence of appropriate nutrients, the E. coli survived within the gelatin matrix for several days. The technique could have a number of potential future applications, including the arrangement of a variety of different cell types in complex architectures, as motifs for promoting tissue differentiation and growth within the field of cell engineering.