Picture of a black hole

Strathclyde Open Access research that creates ripples...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of research papers by University of Strathclyde researchers, including by Strathclyde physicists involved in observing gravitational waves and black hole mergers as part of the Laser Interferometer Gravitational-Wave Observatory (LIGO) - but also other internationally significant research from the Department of Physics. Discover why Strathclyde's physics research is making ripples...

Strathprints also exposes world leading research from the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

Discover more...

Creating permanent 3D arrangements of isolated cells using holographic optical tweezers

Jordan, P. and Leach, J. and Padgett, M.J. and Blackburn, P. and Isaacs, N. and Goksor, M. and Hanstorp, D. and Wright, A.J. and Girkin, J.M. and Cooper, J. (2005) Creating permanent 3D arrangements of isolated cells using holographic optical tweezers. Lab on a Chip, 5 (11). pp. 1224-1228. ISSN 1473-0197

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Reports the creation of permanent 3D configurations of cells, at predefined positions, within a gelatin matrix. The technique used holographic optical tweezers to manipulate individual E. coli within a solution comprising monomer precursors. The matrix was then set and after the laser beam was removed, we were able to demonstrate that the structures remained intact for many days. We were also able to demonstrate that, in the presence of appropriate nutrients, the E. coli survived within the gelatin matrix for several days. The technique could have a number of potential future applications, including the arrangement of a variety of different cell types in complex architectures, as motifs for promoting tissue differentiation and growth within the field of cell engineering.