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Practical implementation of adaptive optics in multiphoton microscopy

Marsh, P.N. and Burns, D. and Girkin, J.M. (2003) Practical implementation of adaptive optics in multiphoton microscopy. Optics Express, 11 (10). pp. 1123-1130. ISSN 1094-4087

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Abstract

A dedicated two-photon microscope incorporating adaptive-optic correction of specimen-induced aberrations is presented. Wavefront alteration of the scanning laser beam was achieved by use of a micromachined deformable mirror. Post scan head implementation produces a compact module compatible with the Bio-Rad MRC-600 scan head. Automatic aberration correction using feedback from the multiphoton fluorescence intensity allowed the adaptive optic to extend the imaging depth attainable in both artificial and biological refractive-index mismatched samples. With a 1.3-NA, x40, Nikon oil immersion objective, the imaging depth in water was extended from approximately 3.4 to 46.2 µm with a resolution defined by a FWHM axial point-spread function of 1.25 µm.

Item type: Article
ID code: 4221
Keywords: optics, photonics, lasers, quantum electronics, Optics. Light, Atomic and Molecular Physics, and Optics
Subjects: Science > Physics > Optics. Light
Department: Faculty of Science > Institute of Photonics
Unknown Department
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 05 Sep 2007
    Last modified: 04 Sep 2014 15:28
    URI: http://strathprints.strath.ac.uk/id/eprint/4221

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