Marsh, P.N. and Burns, D. and Girkin, J.M. (2003) Practical implementation of adaptive optics in multiphoton microscopy. Optics Express, 11 (10). pp. 1123-1130. ISSN 1094-4087Full text not available in this repository. (Request a copy from the Strathclyde author)
A dedicated two-photon microscope incorporating adaptive-optic correction of specimen-induced aberrations is presented. Wavefront alteration of the scanning laser beam was achieved by use of a micromachined deformable mirror. Post scan head implementation produces a compact module compatible with the Bio-Rad MRC-600 scan head. Automatic aberration correction using feedback from the multiphoton fluorescence intensity allowed the adaptive optic to extend the imaging depth attainable in both artificial and biological refractive-index mismatched samples. With a 1.3-NA, x40, Nikon oil immersion objective, the imaging depth in water was extended from approximately 3.4 to 46.2 µm with a resolution defined by a FWHM axial point-spread function of 1.25 µm.
|Keywords:||optics, photonics, lasers, quantum electronics, Optics. Light, Atomic and Molecular Physics, and Optics|
|Subjects:||Science > Physics > Optics. Light|
|Department:||Faculty of Science > Physics > Institute of Photonics|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||05 Sep 2007|
|Last modified:||04 Nov 2016 03:09|