Marsh, P.N. and Burns, D. and Girkin, J.M. (2003) Practical implementation of adaptive optics in multiphoton microscopy. Optics Express, 11 (10). pp. 1123-1130. ISSN 1094-4087
Full text not available in this repository. (Request a copy from the Strathclyde author)Abstract
A dedicated two-photon microscope incorporating adaptive-optic correction of specimen-induced aberrations is presented. Wavefront alteration of the scanning laser beam was achieved by use of a micromachined deformable mirror. Post scan head implementation produces a compact module compatible with the Bio-Rad MRC-600 scan head. Automatic aberration correction using feedback from the multiphoton fluorescence intensity allowed the adaptive optic to extend the imaging depth attainable in both artificial and biological refractive-index mismatched samples. With a 1.3-NA, x40, Nikon oil immersion objective, the imaging depth in water was extended from approximately 3.4 to 46.2 µm with a resolution defined by a FWHM axial point-spread function of 1.25 µm.
| Item type: | Article |
|---|---|
| ID code: | 4221 |
| Keywords: | optics, photonics, lasers, quantum electronics, Optics. Light |
| Subjects: | Science > Physics > Optics. Light |
| Department: | Faculty of Science > Institute of Photonics Unknown Department |
| Related URLs: | |
| Depositing user: | Strathprints Administrator |
| Date Deposited: | 05 Sep 2007 |
| Last modified: | 12 Mar 2012 10:40 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/4221 |
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