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Impact of processing parameters on the haemocompatibility of Bombyx mori silk films

Seib, F Philipp and Maitz, Manfred F and Hu, Xiao and Werner, Carsten and Kaplan, David L (2012) Impact of processing parameters on the haemocompatibility of Bombyx mori silk films. Biomaterials, 33 (4). pp. 1017-1023.

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Abstract

Silk has traditionally been used for surgical sutures due to its lasting strength and durability; however, the use of purified silk proteins as a scaffold material for vascular tissue engineering goes beyond traditional use and requires application-orientated biocompatibility testing. For this study, a library of Bombyx mori silk films was generated and exposed to various solvents and treatment conditions to reflect current silk processing techniques. The films, along with clinically relevant reference materials, were exposed to human whole blood to determine silk blood compatibility. All substrates showed an initial inflammatory response comparable to polylactide-co-glycolide (PLGA), and a low to moderate haemostasis response similar to polytetrafluoroethylene (PTFE) substrates. In particular, samples that were water annealed at 25 °C for 6 h demonstrated the best blood compatibility based on haemostasis parameters (e.g. platelet decay, thrombin-antithrombin complex, platelet factor 4, granulocytes-platelet conjugates) and inflammatory parameters (e.g. C3b, C5a, CD11b, surface-associated leukocytes). Multiple factors such as treatment temperature and solvent influenced the biological response, though no single physical parameter such as β-sheet content, isoelectric point or contact angle accurately predicted blood compatibility. These findings, when combined with prior in vivo data on silk, support a viable future for silk-based vascular grafts.

Item type: Article
ID code: 42101
Notes: Copyright © 2011 Elsevier Ltd. All rights reserved.
Keywords: silk, blood compatibility, Bombyx mori, PLGA, Pharmacy and materia medica, Biomaterials, Bioengineering, Mechanics of Materials, Ceramics and Composites, Biophysics
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Technology and Innovation Centre > Bionanotechnology
Faculty of Engineering > Electronic and Electrical Engineering
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 16 Nov 2012 11:37
    Last modified: 05 Sep 2014 18:54
    URI: http://strathprints.strath.ac.uk/id/eprint/42101

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