McKeating, Kristy S. and Dougan, Jennifer A. and Faulds, Karen (2012) Nanoparticle assembly for sensitive DNA detection using SERRS. Biochemical Society Transactions, 40. pp. 597-602. ISSN 0300-5127Full text not available in this repository. (Request a copy from the Strathclyde author)
SERRS (surface-enhanced resonance Raman scattering) is a vibrational technique, whereby a relatively weak Raman scattering effect is enhanced through the use of a visible chromophore and a roughened metal surface. The direct analysis of DNA by SERRS requires the modification of a nucleic acid sequence to incorporate a chromophore, and adsorption of the modified sequence on to a roughened metal surface. Aggregated metallic nanoparticles are commonly used in the analysis of dye-labelled DNA by SERRS, allowing for detection levels that rival those gained from standard fluorescence-based techniques. In the present paper, we report on how SERRS can be exploited for the analysis of clinically relevant DNA samples. We also report on the ability of nanoparticles to aggregate as the result of a biologically significant event, as opposed to the use of an external charge-modifying agent. The self-assembly of metallic nanoparticles is shown to be a promising new technique in the move towards extremely sensitive methods of DNA analysis by SERRS.
|Keywords:||nanoparticles, DNA detection , SERRS , surface-enhanced resonance Raman scattering, Chemistry|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry|
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|Depositing user:||Pure Administrator|
|Date Deposited:||17 Oct 2012 10:59|
|Last modified:||12 Jun 2013 14:34|
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