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Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS)

Harper, Mhairi M. and Robertson, Barry and Ricketts, Alastair and Faulds, Karen (2012) Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS). Chemical Communications, 48 (75). pp. 9412-9414. ISSN 1359-7345

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Abstract

We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.