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Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS)

Harper, Mhairi M. and Robertson, Barry and Ricketts, Alastair and Faulds, Karen (2012) Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS). Chemical Communications, 48 (75). pp. 9412-9414. ISSN 1359-7345

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Abstract

We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.

Item type: Article
ID code: 41480
Keywords: surface enhanced Raman scattering , DNA , TaqMan assay , SERS, Physical and theoretical chemistry, Materials Chemistry, Surfaces, Coatings and Films, Metals and Alloys, Ceramics and Composites, Chemistry(all), Catalysis, Electronic, Optical and Magnetic Materials
Subjects: Science > Chemistry > Physical and theoretical chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Technology and Innovation Centre > Bionanotechnology
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Depositing user: Pure Administrator
Date Deposited: 16 Oct 2012 12:59
Last modified: 27 Mar 2014 10:35
URI: http://strathprints.strath.ac.uk/id/eprint/41480

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