Lewis, Helen and Perrett, Andrew and Burley, Glenn and Eperon, Ian (2012) An RNA splicing enhancer that does not act by looping. Angewandte Chemie International Edition, 51 (39). 9800–9803. ISSN 1433-7851Full text not available in this repository. (Request a copy from the Strathclyde author)
Do the proteins bound to an enhancer site on pre-mRNA interact directly with the splice site by diffusion (looping), as is generally accepted, or does the intervening RNA play a role (see scheme)? By inserting a PEG linker between an enhancer sequence and alternative splice sites, the interaction of these two elements can be studied. Intervening RNA was essential for the enhancer activity, which rules out the looping model.
|Keywords:||click chemistry, pre-RNA splicing, mRNA, oligonucleotides, splicing enhancers, looping, rna splicing enhancer, Chemistry|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry|
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|Depositing user:||Pure Administrator|
|Date Deposited:||20 Sep 2012 14:16|
|Last modified:||21 Nov 2013 10:16|
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