Poland, S. P. and Wright, A. J. and Cobb, S. and Vijverberg, J. C. and Girkin, J. M. (2011) A demonstration of the effectiveness of a single aberration correction per optical slice in beam scanned optically sectioning microscopes. Micron, 42 (4). pp. 318-323. ISSN 0968-4328Full text not available in this repository. (Request a copy from the Strathclyde author)
In this paper we report the use of adaptive optics to correct for sample induced aberrations in optical microscopy, crucially comparing individual pixel-by-pixel correction against a single correction for an entire optical section. Sample induced optical aberrations in slices of rat brain tissue were corrected with a deformable membrane mirror. Using axial resolution measurements, we demonstrate that a single aberration correction per optical slice achieves around 80% of the maximum possible improvement compared to individual pixel-by-pixel correction in both confocal and multiphoton microscopy. A single aberration correction per depth, compared to pixel-by-pixel aberration correction, significantly decreases scan times and therefore reduces photobleaching and phototoxic effects enabling more rapid microscopy with active aberration correction. The results confirm that the use of a “look-up” table, based upon sample type and depth, may be the most practical way of implementing adaptive optic aberration correction in beam scanning optical sectioning microscopy.
|Keywords:||biological imaging, implementation, system, confocal microscopy, adaptive optics, multiphoton microscopy, aberration correction, Physics, Structural Biology, Cell Biology|
|Subjects:||Science > Physics|
|Department:||Faculty of Science > Physics > Institute of Photonics|
|Depositing user:||Pure Administrator|
|Date Deposited:||25 Jul 2012 10:56|
|Last modified:||22 Mar 2017 12:14|