Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

A demonstration of the effectiveness of a single aberration correction per optical slice in beam scanned optically sectioning microscopes

Poland, S. P. and Wright, A. J. and Cobb, S. and Vijverberg, J. C. and Girkin, J. M. (2011) A demonstration of the effectiveness of a single aberration correction per optical slice in beam scanned optically sectioning microscopes. Micron, 42 (4). pp. 318-323. ISSN 0968-4328

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

In this paper we report the use of adaptive optics to correct for sample induced aberrations in optical microscopy, crucially comparing individual pixel-by-pixel correction against a single correction for an entire optical section. Sample induced optical aberrations in slices of rat brain tissue were corrected with a deformable membrane mirror. Using axial resolution measurements, we demonstrate that a single aberration correction per optical slice achieves around 80% of the maximum possible improvement compared to individual pixel-by-pixel correction in both confocal and multiphoton microscopy. A single aberration correction per depth, compared to pixel-by-pixel aberration correction, significantly decreases scan times and therefore reduces photobleaching and phototoxic effects enabling more rapid microscopy with active aberration correction. The results confirm that the use of a “look-up” table, based upon sample type and depth, may be the most practical way of implementing adaptive optic aberration correction in beam scanning optical sectioning microscopy.

Item type: Article
ID code: 40541
Keywords: biological imaging, implementation, system, confocal microscopy, adaptive optics, multiphoton microscopy, aberration correction, Physics, Structural Biology, Cell Biology
Subjects: Science > Physics
Department: Faculty of Science > Institute of Photonics
Related URLs:
Depositing user: Pure Administrator
Date Deposited: 25 Jul 2012 11:56
Last modified: 27 Mar 2014 10:20
URI: http://strathprints.strath.ac.uk/id/eprint/40541

Actions (login required)

View Item