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Ecotin-like serine peptidase inhibitor ISP1 of Leishmania major plays a role in flagellar pocket dynamics and promastigote differentiation

Morrison, Lesley S and Goundry, Amy and Faria, Marilia S and Tetley, Laurence and Eschenlauer, Sylvain C and Westrop, Gareth D and Dostalova, Anna and Volf, Petr and Coombs, Graham H and Lima, Ana Paula C A and Mottram, Jeremy C (2012) Ecotin-like serine peptidase inhibitor ISP1 of Leishmania major plays a role in flagellar pocket dynamics and promastigote differentiation. Cellular Microbiology. (In Press)

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Abstract

Leishmania ISPs are ecotin-like natural peptide inhibitors of trypsin-family serine peptidases, enzymes that are absent from the Leishmania genome. This led to the proposal that ISPs inhibit host serine peptidases and we have recently shown that ISP2 inhibits neutrophil elastase, thereby enhancing parasite survival in murine macrophages. In this study we show that ISP1 has less serine peptidase inhibitory activity than ISP2, and in promastigotes both are generally located in the cytosol and along the flagellum. However, in haptomonad promastigotes there is a prominent accumulation of ISP1 and ISP2 in the hemidesmosome and for ISP2 on the cell surface. An L. major mutant deficient in all three ISP genes (Δisp1/2/3) was generated and compared with Δisp2/3 mutants to elucidate the physiological role of ISP1. In in vitro cultures, the Δisp1/2/3 mutant contained more haptomonad, nectomonad and leptomonad promastigotes with elongated flagella and reduced motility compared with Δisp2/3 populations, moreover it was characterized by very high levels of release of exosome-like vesicles from the flagellar pocket. These data suggest that ISP1 has a primary role in flagellar homeostasis, disruption of which affects differentiation and flagellar pocket dynamics.

Item type: Article
ID code: 40367
Notes: © 2012 Blackwell Publishing Ltd.
Keywords: Leishmania ISP, Leishmania genome, serine peptidases, Pharmacy and materia medica, Immunology, Virology, Microbiology
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
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Depositing user: Pure Administrator
Date Deposited: 09 Jul 2012 09:58
Last modified: 27 Mar 2014 10:15
URI: http://strathprints.strath.ac.uk/id/eprint/40367

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