Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Oligopeptidase B deficient mutants of Leishmania major

Munday, Jane C. and McLuskey, Karen and Brown, Elaine and Coombs, Graham H and Mottram, Jeremy C (2011) Oligopeptidase B deficient mutants of Leishmania major. Molecular and Biochemical Parasitology, 175 (1). pp. 49-57. ISSN 0166-6851

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Oligopeptidase B is a clan SC, family S9 serine peptidase found in gram positive bacteria, plants and try-panosomatids. Evidence suggests it is a virulence factor and thus therapeutic target in both Trypanosome cruzi and T. brucei, but little is known about its function in Leishmania. In this study L major OPB-deficient mutants (Delta opb) were created. These grew normally as promastigotes, had a small deficiency in their ability to undergo differentiation to metacyclic promastigotes, were significantly less able to infect and survive within macrophages in vitro, but were virulent to mice. These data suggest that L major OPB itself is not an important virulence factor, indicating functional differences between trypanosomes and Leishmania in their interaction with the mammalian host. The possibility that an OPB-like enzyme (designated OPB2) in L major might compensate for the loss of OPB in Delta opb was investigated via by mapping its sequence onto the 1.6 angstrom structure of L. major OPB. This suggested that the residues involved in the S1 and S2 subsites of OPB2 are identical to OPB and hence the substrate specificity would be similar. Consequently there may be redundancy between the two enzymes. (C) 2010 Elsevier B.V. All rights reserved.

Item type: Article
ID code: 40360
Notes: Copyright © 2010 Elsevier B.V. All rights reserved.
Keywords: Leishmania, Peptidase, Virulence, Oligopeptidase, Pharmacy and materia medica, Molecular Biology, Parasitology
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
Depositing user: Pure Administrator
Date Deposited: 06 Jul 2012 16:36
Last modified: 27 Mar 2014 09:16
URI: http://strathprints.strath.ac.uk/id/eprint/40360

Actions (login required)

View Item