t'Kindt, Ruben and Scheltema, Richard A and Jankevics, Andris and Brunker, Kirstyn and Rijal, Suman and Dujardin, Jean-Claude and Breitling, Rainer and Watson, David G and Coombs, Graham H and Decuypere, Saskia (2010) Metabolomics to unveil and understand phenotypic diversity between pathogen populations. PLOS Neglected Tropical Diseases, 4 (11). ISSN 1935-2727
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Leishmaniasis is a debilitating disease caused by the parasite Leishmania. There is extensive clinical polymorphism, including variable responsiveness to treatment. We study Leishmania donovani parasites isolated from visceral leishmaniasis patients in Nepal that responded differently to antimonial treatment due to differing intrinsic drug sensitivity of the parasites. Here, we present a proof-of-principle study in which we applied a metabolomics pipeline specifically developed for L. donovani to characterize the global metabolic differences between antimonial-sensitive and antimonial-resistant L. donovani isolates. Clones of drug-sensitive and drug-resistant parasite isolates from clinical samples were cultured in vitro and harvested for metabolomics analysis. The relative abundance of 340 metabolites was determined by ZIC-HILIC chromatography coupled to LTQ-Orbitrap mass spectrometry. Our measurements cover approximately 20% of the predicted core metabolome of Leishmania and additionally detected a large number of lipids. Drug-sensitive and drug-resistant parasites showed distinct metabolic profiles, and unsupervised clustering and principal component analysis clearly distinguished the two phenotypes. For 100 metabolites, the detected intensity differed more than three-fold between the 2 phenotypes. Many of these were in specific areas of lipid metabolism, suggesting that the membrane composition of the drug-resistant parasites is extensively modified. Untargeted metabolomics has been applied on clinical Leishmania isolates to uncover major metabolic differences between drug-sensitive and drug-resistant isolates. The identified major differences provide novel insights into the mechanisms involved in resistance to antimonial drugs, and facilitate investigations using targeted approaches to unravel the key changes mediating drug resistance.
|Keywords:||Leishmania donovani promastigotes , LC-MS data, pentamidine resistant Leishmania, in-vitro sisceptibility, visceral Leishmaniasis, mass-spectrometry, amphotericin-b, lipid-metabolism, genes, Pharmacy and materia medica, Infectious Diseases, Public Health, Environmental and Occupational Health, Pharmacology, Toxicology and Pharmaceutics(all)|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Pure Administrator|
|Date Deposited:||06 Jul 2012 15:23|
|Last modified:||26 Mar 2015 17:19|
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