Somasundaram, B. and Hoyland, J. and Dempster, John and Riley, J. and Mason, W.T. (1992) Simultaneous patch-clamp and photometric fluorescence ratio measurements of optical probes for ions using a computer-based data acquisition system, PhoCal. Journal of Physiology, 446 (Suppl.). P63-P63. ISSN 0022-3751Full text not available in this repository. (Request a copy from the Strathclyde author)
This demonstration will present a new computer program and analytical system - PhoCal - which permits the simultaneous measurement of (a) low light levels of fluorescence from optical probes for ions and (b) up to two channels of physiological data which can include patch-clamp current and voltage, or voltage from other probes such as extracellular pH electrodes or other transducers and (c) an event marker channel. The system is PC-based and uses high speed analogto-digital circuitry operating in parallel with a two-channel photon counter which collects data from one or two photomultiplier tubes. All data is logged directly to hard disk and the system can operate at speeds in excess of an aggregate of 1000 samples per second.
|Keywords:||patch clamp, photometric fluorescence, PhoCal, Pharmacy and materia medica, Physiology|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Pure Administrator|
|Date Deposited:||29 Jun 2012 10:25|
|Last modified:||04 May 2016 22:06|