Picture of a sphere with binary code

Making Strathclyde research discoverable to the world...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. It exposes Strathclyde's world leading Open Access research to many of the world's leading resource discovery tools, and from there onto the screens of researchers around the world.

Explore Strathclyde Open Access research content

Simultaneous patch-clamp and photometric fluorescence ratio measurements of optical probes for ions using a computer-based data acquisition system, PhoCal

Somasundaram, B. and Hoyland, J. and Dempster, John and Riley, J. and Mason, W.T. (1992) Simultaneous patch-clamp and photometric fluorescence ratio measurements of optical probes for ions using a computer-based data acquisition system, PhoCal. Journal of Physiology, 446 (Suppl.). P63-P63. ISSN 0022-3751

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

This demonstration will present a new computer program and analytical system - PhoCal - which permits the simultaneous measurement of (a) low light levels of fluorescence from optical probes for ions and (b) up to two channels of physiological data which can include patch-clamp current and voltage, or voltage from other probes such as extracellular pH electrodes or other transducers and (c) an event marker channel. The system is PC-based and uses high speed analogto-digital circuitry operating in parallel with a two-channel photon counter which collects data from one or two photomultiplier tubes. All data is logged directly to hard disk and the system can operate at speeds in excess of an aggregate of 1000 samples per second.